ML Command-Line Reference
Visual Studio .NET 2003
Assembles and links one or more assembly-language source files. The command-line options are case sensitive.

ML [[options]] filename [[ [[options]]  filename]]
...
[[/link linkoptions]]

filename 
    The name of the file. 
linkoptions 
    The compiler link options.

Parameters:
    /AT                 Enables tiny-memory-model support. Enables error messages for code constructs that violate the requirements for .com format files. Note that this is not equivalent to the .MODEL TINY directive. 
    /Bl <filename>      Selects an alternate linker. 
    /c                  Assembles only. Does not link. 
    /coff               Generates common object file format (COFF) type of object module. Generally required for Win32 assembly language development. 
    /Cp                 Preserves case of all user identifiers. 
    /Cu                 Maps all identifiers to upper case (default). 
    /Cx                 Preserves case in public and extern symbols. 
    /D<symbol[[=value]]>Defines a text macro with the given name. If value is missing, it is blank. Multiple tokens separated by spaces must be enclosed in quotation marks. 
    /EP                 Generates a preprocessed source listing (sent to STDOUT). See /Sf. 
    /F <hexnum>         Sets stack size to hexnum bytes (this is the same as /link /STACK:number). The value must be expressed in hexadecimal notation. There must be a space between /F and hexnum. 
    /Fe <filename>      Names the executable file. 
    /Fl[[filename]]     Generates an assembled code listing. See /Sf. 
    /Fm[[filename]]     Creates a linker map file. 
    /Fo <filename>      Names an object file. 
    /FPi                Generates emulator fix-ups for floating-point arithmetic (mixed language only). 
    /Fr[[filename]]     Generates a source browser .sbr file. 
    /FR[[filename]]     Generates an extended form of a source browser .sbr file. 
    /Gc                 Specifies use of FORTRAN- or Pascal-style function calling and naming conventions. Same as OPTION LANGUAGE:PASCAL. 
    /Gd                 Specifies use of C-style function calling and naming conventions. Same as OPTION LANGUAGE:C. 
    /H <number>         Restricts external names to number significant characters. The default is 31 characters. 
    /help               Calls QuickHelp for help on ML. 
    /I <pathname>       Sets path for include file. A maximum of 10 /I options is allowed. 
    /nologo             Suppresses messages for successful assembly. 
    /omf                Generates object module file format (OMF) type of object module. 
    /Sa                 Turns on listing of all available information. 
    /safeseh            Marks the object as either containing no exception handlers or containing exception handlers that are all declared with .SAFESEH. 
    /Sc                 Adds instruction timings to listing file. 
    /Sf                 Adds first-pass listing to listing file. 
    /Sg                 Turns on listing of assembly-generated code. 
    /Sl <width>         Sets the line width of source listing in characters per line. Range is 60 to 255 or 0. Default is 0. Same as PAGE width. 
    /Sn                 Turns off symbol table when producing a listing. 
    /Sp <length>        Sets the page length of source listing in lines per page. Range is 10 to 255 or 0. Default is 0. Same as PAGE length. 
    /Ss <text>          Specifies text for source listing. Same as SUBTITLE text. 
    /St <text>          Specifies title for source listing. Same as TITLE text. 
    /Sx                 Turns on false conditionals in listing. 
    /Ta <filename>      Assembles source file whose name does not end with the .asm extension. 
    /w                  Same as /W0. 
    /W<level>           Sets the warning level, where level = 0, 1, 2, or 3. 
    /WX                 Returns an error code if warnings are generated. 
    /Zd                 Generates line-number information in object file. 
    /Zf                 Makes all symbols public. 
    /Zi                 Generates CodeView information in object file. 
    /Zm                 Enables M510 option for maximum compatibility with MASM 5.1. 
    /Zp[[alignment]]    Packs structures on the specified byte boundary. The alignment can be 1, 2, or 4. 
    /Zs                 Performs a syntax check only. 
    /?                  Displays a summary of ML command-line syntax. 

Environment Variables:
    INCLUDE     Specifies search path for include files. 
    ML          Specifies default command-line options. 
    TMP         Specifies path for temporary files. 

MSDN Link